Conférence scientifique du vendredi midi - Rasim Barutcu

Systematic Mapping of Nuclear Domain‐RNA Associations to Discover Novel Regulatory Mechanisms

vendredi 17 juin 2022 de 12:00 à 13:00

Pour l'axe Maladies immunitaires et cancers

Résumé

The nucleus is highly compartmentalized through the formation of distinct classes of sub-nuclear domains, which are involved in the regulation of several nuclear functions, such as transcriptional control or splicing.

Therefore, dysregulation of these structures is highly implicated in several diseases, especially in cancer. Importantly, many therapeutic drugs used in cancer treatments are localized within specific nuclear domains, as opposed to evenly diffusing out within the cell. Despite decades of work, the composition and function of many of these structures is not well understood. Using APEX2-mediated proximity labelling and RNA sequencing, we surveyed transcripts associated with nuclear speckles, several additional domains, and the lamina.

Remarkably, speckles and lamina are associated with distinct classes of retained introns enriched in genes that function in RNA processing, translation, and the cell cycle, processes often perturbed in cancer. In contrast to the lamina-proximal introns, retained introns associated with speckles are relatively short, GC-rich, and enriched for functional sites of RNA binding proteins that are concentrated in these domains. They are also highly differentially regulated across diverse cellular contexts, including the cell cycle.

Our study thus provides a rich resource of nuclear domain-associated transcripts, opening new ways of thinking about intron retention and cell cycle regulation, with implications for cancer research.

Présentée par

• Rasim Barutcu, PhD
  • Postdoctoral Fellow, University of Toronto

Date et Lieu

Vendredi, 17 juin 2022 12 h à 13 h

En présentiel : Amphithéâtre 125

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